ascorbic acid, Beta propilacton and binary ethylenamine (BEA) were evaluated as inhibitors of rabies' virus (Evely- Retkitincki-Abeleseths skauis) in optimized BHK21 cell cultures. Ascorbic acid inactivated rabies virus at concentrations of 0.1, 0.5 and1mg/ ml after 22,18 and 14 hours at 37o C respectively, while BPL inactivated the virus after 4,6,8 hours with concentrations of 1/500,1/10000,and1/20000 respectively at 37oC. BEI inactivated the virus after 6 hours when used with 0.3 M. These Inactivators did not affect the antigincity of rabies virus in three prepared experimental vaccine batches. Ascorbic acid appears to be the most preferable one as safe natural noncarcinogenic agent when compared with BPL and BEI.