This study has been designed as a trial for comparing of ISA206 adjuvanted Foot and Mouth Disease (FMD) vaccine (commercial vaccine), commercial FMD vaccine containing vitamin (A), commercial FMD vaccine containing vitamin (ESelenium), and commercial FMD vaccine containing both vitamin (A) and vitamin (E-Selenium) together in eliciting immunity. The obtained results revealed that the duration of humoral immunity elicited by commercial vaccine only was 30 weeks, while, it was 32 weeks in a commercial vaccine containing vitamin (A), 36 weeks in a commercial vaccine containing vitamin (E-Selenium) and 38 weeks in a commercial vaccine containing both vitamin (A) and vitamin (ESelenium) together in vaccinated sheep. Results of cellular immunity evaluation indicated that mean optical density (mean OD) correspond to non-specific mitogens using MTT kit reached its highest values by 21st-day post-vaccination in all vaccinated sheep and mean OD values correspond to FMDV as specific mitogen were higher than that of non-specific mitogen. Also, the highest mean OD values were obtained in vaccinated sheep with a commercial vaccine containing both vitamin (A) and vitamin (E-Selenium) together. It could be concluded that usage of inactivated oil FMD vaccine using Montanide ISA 206 containing both vitamin (A) and vitamin (E-selenium) resulted in longer-lasting immunity than that with Montanide ISA 206 only, besides the stimulating effect of both vitamin (A) and vitamin (E-selenium) in both humoral and cellular immunity.