The present study aimed to estimate the chemical components and cytotoxic effects of four types of wastewater such as industrial wastewater (IWW), agriculture wastewater (AWW), sewage wastewater (SWW) and mixed wastewater (MWW) on meristematic tissues and cells of faba bean, (Vicia faba) root tips. Some physio-chemical characteristics as well as heavy metals contents were measured in these four wastewater samples compared with that of tap water using values of the World Health Organization (WHO) as standard parameters. Mitotic index (MI), phase index and chromosomal abnormalities were calculated as toxicity indicators. The results revealed that MI values of almost all seeds treated with the different concentrations of various types of wastewater were significantly lower than those of control treatment. Different types of mitotic aberration were observed such as chromosome bridges, lagging chromatid and chromosomes, chromatin fragments, micronuclei and tripolar cells. It was clearly observed that wastewater increased chromosomal aberrations and induced disturbances in mitotic phases. Therefore, it could be recommended that all wastewater should be treated according to WHO standards to avoid and minimize the pollutant effects before using for irrigation purpose so that safety of humans would be achieved.