Calotropis procera latex treatments against Locusta migratoria were very persuasive due to mortality efficiency and haemolymph contents of treated nymphs. This study showed the impact of five Usher latex doses 10, 20, 30, 40, and 50 µl by topical applications on 3^rd, 4^th and 5^th. The Ld₅₀ values were 10.3, 10.9 and 12.2µl respectively. The effect of latex usher concentration LD₅₀ (12.2 µl) on haemolymph contents, total carbohydrates, total lipids, total protein and cholesterol of 5^th nymphal instar were carried out and  showed  that the haemolymph content of the treated insects was highly affected and all the studied parameters were lower than the control.