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118061

Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum

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Last updated: 04 Jan 2025

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Abstract

Bacterial wilt of potatoes (Solanum tuberosum) caused by Ralstonia solanacearum phylotype II sequevar I (race 3 biovar 2), is one of the most serious diseases of potatoes. This study aimed to detect such deteriorations through the remotely sensed data. In this regard, naturally infested potato fields at Abo Alkhawi, Al Tayareya and Om Saber villages at Behera governorate, Egypt, were used for selective isolation of R. solanacearum. On Semi Selective South Africa (SMSA) medium, 30 isolates were randomly screened and tested by immunofluorescence antibody staining (IFAS) and confirmed biologically by tomato plantlets (cv. Pinto) reaction. All isolates showed positive results with Real-time PCR. Based on the phylotype specific multiplex (Pmx) – PCR data, all isolates in concern were R. solanacearum and affiliated to phylotype II as 372- bp amplicon.
Spectral indices were applied on the Landsat 8 and RapidEye satellite images acquired in January and April 2019 including Normalized Difference Moisture Index (NDMI), Normalized Difference Vegetation Index (NDVI), Normalized Difference Salinity Index (NDSI) and Modified Chlorophyll Absorption Ratio Index (MCARI). Results from the remote sensing analyses showed medium to relatively high salinity and moisture content of soils concerened. Moreover, plants chlorophyll levels are in medium levels which is not typical to condition associated with usual circumstances. Further investigation, in a greater academic depth, are needed for pathological field surveys.
 

DOI

10.21608/ejar.2020.118061

Keywords

SMSA medium, immunofluorescent antibody staining (IFAS), Phylotype analysis, Real-Time PCR, Wilt and brown rot diseases, Indices, Landsat 8, RapidEye, Soil salinity, moisture content and chlorophyll content

Authors

First Name

NAGLAA

Last Name

BALABEL

MiddleName

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Affiliation

Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt. Potato Brown Rot Project (PBRP), Ministry of Agriculture and Land Reclamation, Dokki, Egypt.

Email

nbalabel@yahoo.com

City

-

Orcid

-

First Name

Ali

Last Name

Mamdouh

MiddleName

M.

Affiliation

Geology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt.

Email

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City

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Orcid

-

First Name

Shams

Last Name

Younes

MiddleName

Nour El-Dein

Affiliation

Department of Geography, Faculty of Arts, Cairo University, Giza, Egypt.

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Volume

98

Article Issue

2

Related Issue

17767

Issue Date

2020-07-01

Receive Date

2020-07-10

Publish Date

2020-07-01

Page Start

240

Page End

269

Print ISSN

1110-6336

Online ISSN

2812-4936

Link

https://ejar.journals.ekb.eg/article_118061.html

Detail API

https://ejar.journals.ekb.eg/service?article_code=118061

Order

4

Type

Original Article

Type Code

1,041

Publication Type

Journal

Publication Title

Egyptian Journal of Agricultural Research

Publication Link

https://ejar.journals.ekb.eg/

MainTitle

Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum

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Article

Created At

22 Jan 2023