The immunogenicity and clinical safety of a local live attenuated canine adenovirus-1 vaccine (CAV-1) were evaluated in experimentally vaccinated native breed 3-4 months puppies. All vaccinated puppies did not show any abnormalities post-vaccination. Studying the blood picture revealed no changes in RBCs and haemoglobin values while there was an increase in the levels of total WBCs count, lymphocyte %, total serum protein and serum globulin. Liver and kidney function tests results were found to be within the normal values. These findings ensure vaccine safety. On the other side, the serum neutralization test was carried out on sera of vaccinated puppies and showed that these animals exhibited good levels of specific CAV-1 serum neutralizing antibodies able to protect them against virus infection. So, it could be said that the local CAV-1 vaccine is immunogenic vaccine suitable for dog protection against infectious canine hepatitis.