Evaluation the effect of biochemistry and toxicity by different concentrations of photosensitizer compound against full grown larvae of B. zonata . Treatments were evaluated under laboratory conditions. The results revealed that, chlorophyllin taken by Bactrocera zonata , showed low toxicity under severe dark and light-based toxic conditions when insects were exposed to light. Fruit fly after processing by photosensitizer compound (magnesium chlorophyllin) and exposure to sunlight for two hour was more effective than photosensitizer (copper chlorophyllin) at same conditions, Sub-lethal concentrations (LC50) (0.00002 M, 0.008 M), respectively. Bioassays show significant differences and good correlation found between the toxicity results and biochemical assays in our study give highest increase of activity level in total carbohydrate and on the other hand, total lipid and total Protein show variation reduction in most treatments.