Chlorophyllin (CHL) is water-soluble analogue of chlorophyll pigment everywhere. This is due to the presence of many useful properties and its use as food additives and in alternative medicine. The study field was investigational at station of the faculty of Agricultural, Cairo University, at Giza governorates, Egypt. Result indicated that sub-lethal concentrations of sodium copper chlorophyllin and magnesium copper chlorophyllin on field and susceptible strains Spodoptera littoralis . The concentration (10-2 M) for both sodium copper chlorophyllin and magnesium copper chlorophyllin was more effective than concentration (10-3 M) on field strains and susceptible strains of Spodoptera littoralis. The determination activity level α-esterases, βesterases and acetylcholine were determined in the total body homogenates of Spodoptera littoralis . The results show that CHL was affected both (α – β) esterase enzyme. β –esterase activity showed significant activity increase, and α-esterase showed significant activity reduction in most treatment. on the other hand the acetylcholinesterase activity show low various.