Background: Universities and colleges are increasingly using e-learning as a delivery method. Although a growing number of educational institutions are adopting an online teaching and learning approach, some concern has been expressed about the pre-requisites for academic success within this environment. This study aimed to assess thecorrelation between perception and psychological challenges toward e-learning among Faculty of Nursing Students. Subjects and method: A descriptive research design was used in this study. A Stratified sample was used. The present study included 341 students. Setting: This study was carried out at the Faculty of Nursing- Assiut University. Tools of study: Included demographic data, Students' Perceptions of e-learning and Psychological challenges of e-learning questionnaire. Results of the study: About two thirds of studied students in the age group (from 18-20 yrs old) are females and less than half of them prefer hybrid mode for their learning. Also, less than of nursing students have a negative perceptions regarding e-learning, and more than one third are unmotivated and unsatisfied from e-learning. Conclusion: The students' perception regarding e-learning negatively correlated with anxiety, while positively correlated with motivation and satisfaction with e-learning. Recommendations: Psychoeductional programs can be designed and administered to nursing students to improve perception and overcome their psychological challenges toward e-learning.