Background: COVID-19 is an international public health challenge; so, it is crucial to improve patients' knowledge and adherence to the preventive measures of COVID-19 to lower the chance of its transmission. Aims: to assess patient's knowledge and adherence to the preventive measures of COVID-19 at Assiut Chest Hospital and Suggested Nursing Brochure. Research design: A descriptive design was utilize. Setting: Emergency unit and departments at Assiut Chest Hospital. Sample: 220 patients admitted to Assiut Chest Hospital with COVID-19. Tools: An interview Questionnaire and Adherence to preventive measures. Results (82.3%) of the studied patients had poor knowledge about COVID-19, while (94.5%) had inadequate adherence to the preventive measures of COVID-19. Conclusion: It was concluded that COVID-19 patients had poor knowledge and inadequate adherence to the preventive measures of COVID-19. Recommendations: providing a simplified illustrated and comprehensive Arabic brochure containing an information about COVID-19 and its preventive measures.