Background: Due to the immunosuppressive nature of pregnancy, severe sickness and COVID-19 infection are often more likely to affect pregnant women. It has been advised to take many safety measures to prevent being exposed to the virus. The preparedness to embrace public health initiatives is greatly influenced by knowledge and behavior regarding COVID-19. Aim: Analyze the level of knowledge and practices of pregnant women regarding COVID-19 prevention. Methods: A descriptive design was used in the current study. Setting: The present study was conducted at antenatal outpatient clinics in Sohag University Hospital. Subjects: 350 pregnant women who attended to antenatal outpatient clinics were included in the in the study. Tools of data collection:  Data collection was obtained by using A structured interviewing questionnairethat involved three parts (personal data, knowledge assessment sheet and practices assessment sheet). Results: Majority of the studied women had satisfactory practices, whereas more than two thirds of them had inadequate knowledge regarding COVID-19 prevention. Conclusion:  Pregnant women have insufficient understanding of COVID-19 and poor preventive behaviors. Recommendations: Establish educational initiatives to improve women's awareness of and behavior toward COVID-19.