The aim of the study was to evaluate the effect of applying teaching protocol for nurses and lab technicians about infection control measures for patients undergoing dental extraction. Research design: Quasi experimental (pre \ posttest) research design was utilized. Setting: The study was conducted in Faculty of Dentistry at Al-Al-Azhar university outpatients‘ clinic Assiut Branch. Subjects: A convenience sample of all available nurses and lab technicians (36) participating in the study Tools: Tool I: Nurses and lab technicians assessment questionnaire, Tool II: Observation checklist about dental infection control measures. Results: There was high statistical significance difference between knowledge and practice pre and post application of the teaching protocol with p-value equal (<0.001**).Conclusion: nurse and lab technician's knowledge and practice improved post implementation of the teaching protocol about infection control measures for patients undergoing dental extraction. Recommendations: nurse and lab technician need continues education and in-service training programs to be well organized within thedental part in Al –Azhar hospital