Background: Shaken baby syndrome (SBS) is an extremely problematic form of physical violence endured by newborn infants. It is resulting in brain trauma and probably eventual neurological squeals. Despite its danger, it can be prevented by improving the mothers' performance, which includes their knowledge, attitudes and beliefs regarding prevention of SBS in a very simple way. Aim: evaluate the effect of an educational guideline program on mothers' performance regarding shaken baby syndrome prevention. Design: A quasi experimental design was utilized. Sample: A purposive sample of 150 mothers who confessed to inpatient and outpatient pediatric and obstetric departments at Beni-Suef University Hospital. Tool of data collection: three tools were utilized: Tool 1: mothers' interviewing questionnaire for Sociodemographic characteristics. Tool 2: mothers' knowledge about SBS. Tool 3: adopted mothers' beliefs and attitudes regarding Shaking Baby Syndrome prevention sheet (pre/post and follow up tests). Results: Increased Percentage distribution of total mothers' performance (knowledge, attitudes and beliefs) after educational guideline program implementation regarding shaken baby syndrome prevention. Conclusion: Mothers' performance regarding the prevention of shaking baby syndrome improved after implementation of the educational guideline program. Recommendation: Routinely services awareness to avoid shaking baby syndrome in all counseling programs for new mothers' preparation and provide them with brochures on how to deal with an angry infant especially throughout the first months of life.