Education and training of staff are important elements to create safety measures as potential contributing factors to risks and hazards of health care associated injury or harm and as intervention used in eliminating or preventing harm. Aim: to improve knowledge and practices of nurses and technicians about radiation safety measures. Research design: Quiz-experimental research design was used . Setting: It was conducted in Main Assuit University hospital. Sample: 105 nurses and technicians selected randomly. Tools: Two tools was used, self-administrative questionnaire and observation check list. The study was carried through 3 stages: preparatory, implementation and evaluation stage. Results: It showed that 83.8 % of studied participants had poor score of knowledge about radiation safety measures in pretest with improvement in post-test. Also there was statistical significant difference (P-value=0.000) between mean score of pre, post and follow up tests of Radiation Safety Measures program. Conclusion: Majority of the studied sample having poor score of knowledge and practice before in service program and improvement in their knowledge and practice occur in posttest and follow up after implementation the program. Recommendations: Periodic in –service training program for nurses and technicians about radiation safety measures should be done.