Background & Objectives: Preconception health includes health of woman and man before pregnancy to reduce risks that woman may face during pregnancy and enhance fetal and maternal outcomes. The present study aimed to evaluate the effect of the educational program on knowledge and attitude of adolescents nursing students regarding preconception care. Methodology: Quasi-experimental research design was applied in this study. The current study was conducted in Technical Institute of Nursing, Assiut University and Technical Institute of Nursing Al-Azaher University. The studied students were selected by simple random sample. Data collection was done using structured questionnaires which were distributed to the participants. An educational program was implemented to enhance students' knowledge and their attitude about preconception care. Data analysis was done using SPSS program, version 23. Results: A total of 200 students were enrolled in the present study, aged (18-19) years with mean ±SD (18.48±0.584) and 85.0% of them were female. Regarding students' knowledge score was 26.40±11.39 pre education while after implemented the education program become 40.74±10.56 with highly significant differences (P value= <0.001). Moreover, mean score of the students' attitude had 12.37±1.74 regarding preconception care while after the application of the educational program had become 15.14±1.55. Conclusion: There was a relation between total score of students' knowledge regarding preconception care with student's age, gender and university grade, moreover, there was a positive correlation between students' knowledge and their attitude. Recommendation: Further studies are needed using large samples to raise awareness of students about preconception care.