Chronic kidney diseases affect up to one in ten adults globally, and it is always permanent and progressive. Aim: To determine the effect of educational program regarding self-care behavior for hemodialysis patients at Beni-Suef University Hospital. Subject and methods: A quazi-experimental research design has been used. It was conducted at Beni-Suef University Hospital. 55 patients who attended the dialysis unit at Beni-Suef university hospital were selected to participate in the current study. The first tool: A structured interviewing questionnaire that includes two parts; Part 1: Their socio-demographic characteristics. Part 2: include past history of hemodialysis patients. Part 3: Knowledge assessment data sheet used to assess knowledge about kidney and renal failure. Second tool: Self-care measurement scale was used to measure practice regarding self-care behavior of hemodialysis patients. Results: The mean age of the participants was 32.60 ± 20.88, 90.9% of studied sample had poor scores of knowledge at pretest which improved to good scores at immediate posttest. The total universal self-care practice scores of the studied hemodialysis was 75.82 ± 9.90 at pre-test which improved at post and follow-up tests to (100.27 ± 14.02 and 94.38 ± 11.65) respectively, while the total health deviation self-care practices mean scores of the studied hemodialysis was 59.13 ± 6.01 at pre-test which improved in post and follow-up tests to 83.67 ± 6.27 and 80.95 ± 5.25 respectively. Conclusion: The implemented program about self-care behavior had a good impact on hemodialysis patients' knowledge and practice. Recommendations: Dietary counseling and dietary education programs should be held for hemodialysis patients, which promote hemodialysis patients dietary intakes of appropriate food.