Back ground: Quality orientation is a crucial tool for achieving and maintaining competitive advantage. Therefore, firms develop and enhance quality of their human resources through appropriate trainings and development initiatives. Aim: Explore the effect of quality orientation educational program on nursing staff loyalty. Design: Quais experimental design was used. Setting: The research was carried out at South Egypt Cancer Institute. Subject: Quota sample of nursing personnel. Methods: three data collection tools: Personal data of the research subject. Quality orientation questionnaire, Employees loyalty questionnaire. Results: the majority of nursing staff had poor mean score of knowledge in pre- program assessment but this mean score improved in the post-program and followup phase. As regard quality orientation and employee loyalty in preprogram assessment but improvement knowledge in immediate post and follow up phase. Conclusion: There were statistically significant variations in quality orientation and employee loyalty between pre& post and pre & follow up phases. Recommendations: Refreshing training courses about quality orientation should provide periodically, to help staff nurse acquainted with the new aspect of quality and encourage the nursing staff to improve and update their knowledge by reading.