Background: Radiation is a health danger in the work setting and in the whole environment. The exposure to ionizing radiation increases causing potential health hazards for patients and staff. Compliance with using healthcare and safety guidelines may help reduce the incidence of health-related hazards sequences. Aim of the study: Assess knowledge, practice and use the safety measures of nurses and technicians regarding radiation hazards. Research design: Description Cross sectional research design was used. Setting: It was conducted in Main Assuit University hospital. Sample: Random 105 nurses and technicians. Tools: Two tools was used, self-administrative questionnaire and observation check list. Result: 83.8% of nurses and technicians had poor knowledge and 68.6 % of them had poor practice regarding radiation hazards and its safety measures Conclusion: The majority of the nurses and technicians had poor knowledge regarding radiation hazards while more than two thirds of them having poor score of practice. Recommendations: Health educational program for nurses and technicians to improve their knowledge and practice about occupational hazard of radiation and preventive measure