Introduction: Autism Spectrum Disorder (ASD) is a chronic neurodevelopmental disorder with early developmental signs. Globally, about 1 of 68 children have been diagnosed with ASD. Parents of children with ASD face several challenges that can significantly affect their quality of life (QOL) and they must have an acceptable level of emotional intelligence to provide appropriate care. Aim: To evaluate the association between emotional intelligence, QOL, and the burden among mothers of children with ASD. Methods: Correlational descriptive design was used to recruit a convenience sample of 140 mothers of children with ASD, who were visiting the Psychiatric Health Hospital in Port Said, Egypt. Four tools were used to collect the data from the mothers, which are including sociodemographic sheet, burden assessment schedule (BAS), Wong and Law emotional intelligence scale (WLEIS), and world health organization QOL assessment-brief (WHOQOL-BREF). Results: The means for total scores (± SD) for the four tools were as follows: emotional intelligence scale (27.95 ± 6.30), WHOQOL-BREF) (45.62 ± 7.55), and BAS (95.11±13.64). The findings indicate a high positive correlation between mothers' emotional intelligence and QOL, and high negative correlation between mothers' emotional intelligence and burden scale, and a high negative correlation between mothers' QOL and burden scale. Conclusion: The emotional intelligence plays an important positive role in improving the burden level and QOL among the mothers of children with ASD. Recommendation: Continuing health education programs should be developed for mothers of children with ASD about the techniques for improving emotional intelligence to decrease burden level and improve their QOL