The inflammatory bowel disease (IBD) nurse specialitis plays a key role in the management of IBD patients who undergoing biological therapy. Aim: Assess the effect of educational program about biological therapy on nurses' performance in caring of patient with IBD and its activity grade. Subjects and methods: All nurses (40) in the Tropical Medicine and Gastroenterology department and outpatient clinic at Assiut University Hospitals, in addition to a sample of 80 IBD patients (forty patient with crohn's disease and another forty patient with ulcerative colitis) who underwent biological therapy, all were assessed before and after the implementation the nursing educational program. Tools: Tool 1: Structured questionnaire to assess nurses` demographic data and knowledge about biological therapy, Tool 2: observation checklist for nurses` practice. Tool3: Patient disease activity scales, and Nursing educational program about biological therapy administration. Results: It found that a highly statistically significant improvement in nurses' knowledge & practice scores after implementing the nursing educational program P<0.001. There was a highly statistically significant difference regarding the IBD activity grade in the studied patients' pre/post program implementation (p < 0.001). Conclusion: The nursing educational program had a highly statistically significant positive effect on nurses' performance and promoting IBD activity grade of patients following the biological therapy administration. Recommendations: Nurses should have a written clinical booklet on safe biological therapy administration is recommended to be established.