Background: Nutritional management of premature neonates is regarded as a necessary and significant aspect of their care. Proper preterm neonatal positioning is necessary for physiological stability and nutritional status of neonates. So, this study aimed to evaluate the effect of different positions on gastric residual volume of preterm neonates in Neonatal Intensive Care Unit (NICU). Research design: A quasi-experimental research design was used. Sample. A Purposive sample of (60) preterm neonates who met the inclusion criteria at NICU of Assiut University Children Hospital. Two tools were utilized to collect the relevant data, Simple questionnaire sheet and Gastric residual volume record sheet. Results of the present study revealed that minimum gastric residual volume was prone position and maximum gastric residual volume was supine position. There was highly statistical significance difference between three different neonatal positions (right lateral, prone and supine) and amount of gastric residual volume. The study concluded that prone and right lateral positions had lower gastric residual volume than supine position in preterm neonates. So, the researcher recommended that periodical educational training programs for NICU nurses are required to keep nurse's knowledge up to date and to ensure that they had effective practice regarding proper position after feeding for preterm neonates.