Abstract Aim: to assess patient caregiver's knowledge and practice toward safe handling chemotherapy, development of suggesting guidelines for patient caregivers toward safe handling chemotherapy. Methods: the study was conducted in oncology unit at Assiut University hospitals. Data were collected from all patient caregiver, convenience sample of patient caregivers (60) male and female working in oncology department, in period from preparation until 72 hour post administration, it consist of (20) nurses, (20) worker and (20) patient family member. Tool utilize for data collection were an Arabic structured interview questionnaire sheet and observation checklist for patient caregivers. Results: there was lack of nurse's knowledge about chemotherapy with percentage (95%). In addition workers had low knowledge about chemotherapy with percentage (50%, 85%, 65% and 45% respectively). Family member had low knowledge regarding knowledge about cancer and chemotherapy at high percentage with (85%, 100% and 80 respectively) and there is lack of practice related to some of equipment is not applicable. Conclusions: majority of patient caregivers had deficient in knowledge and practice towered safe handling chemotherapy. Recommendation: focusing on more training and education for patient caregivers and health monitoring to prevent or reduce risk of exposure