Abstract Background: The awareness of human rights in reproductive health and the lack of adequate warning may owing to many hazards. One of the best times for preparing for these problems is pre-marriage. Aim: This study aimed to evaluate the effects of premarital orientation program on the female adolescent's awareness about sexual and reproductive health. Design: A quasi- experimental, pre-test and post-test one-group only design was utilized in this study. Setting: this study was conducted at the international conference hall of the Fayoum University. Sample: A purposive sample of 180 female adolescents living in rural villages at Fayoum governorate, planning and coming to marriage were recruited in this study. Data collection tool: Data were collected by an interview questionnaire used in two stages of pre and post program implementation. Results: the current study findings illustrated that the total mean score of correct answers was significantly increased among participants after receiving program. Conclusion & recommendations: Premarital orientation program was p.0.001 successful in attaining its aim of positively changing the knowledge of participant's female adolescent. So recommend to eestablish continuous educational training program among health care providers responsible on premarital counseling clinics in rural health center about adolescent's reproductive and sexual health issues.