Background: Academic clinical nurse educators'self-efficiency is described as their judgment of their organizational capacity as well as ability to execute actions needed to achieve the stated output level. Aim: This research aimed to assess self- efficacy of academic clinical nurse educators to assume the roles of preceptors for nurse students. Methods: The study conducted in the faculty of nursing, using a descriptive cross-sectional design. Subjects: Consisted of (62) academic clinical nurse educators from different departments. One tool was used to collect the present study data. It contains five tasks; Role model, Educator, Socializer, Evaluator and Preceptor role. Results: More than two thirds of academic clinical nurse educators had high self-efficacy related to the roles through all tasks of the preceptors. Around one third of them had low self- efficacy related to the role of preceptors as educator, evaluator, and preceptor role. Conclusion: the current study illuminated the need to conduct preceptorship program frequently to prepare ACNEs to act as preceptors in clinical education. Additionally, there is disparity of levels of self-efficacy among ACNE to assume the roles of preceptors.