Abstract Tympanoplasty is a surgical technique to repair a defect in the tympanic membrane with the placement of a graft. Aim: To evaluate the effect of the designed nursing guidelines on outcomes of patients undergoing tympanoplasty. Research design: Quasi-experimental research design. Setting: Otolaryngology department and Outpatient Clinics at Assiut University Hospital. Patients: Sixty adult patients undergoing tympanoplasty were distributed into two equal groups (study and control) 30 patients for each. Tools: Tool I: Patient Questionnaires Sheet, Tool II: Hearing Handicap Inventory for Adults (HHIA) Scale. Results: Study group patients showed highly statistically significant improvement in their level of knowledge as well as social and emotional responses of hearing handicap than the control group (P<0.01). Conclusion: the designed nursing guidelines for patients undergoing tympanoplasty had a significant effects on improvement of patients` knowledge as well as social and emotional responses of hearing handicap. Recommendations: Teaching materials about pre and postoperative and home instructions should be available in Otolaryngology for patients undergoing tympanoplasty.