Brucellosis is a serious community health problem and endemic disease in Egypt in both animal and human.Community health nurse had vital role to prevent & control of brucellosis. Aims: to evaluate the effectiveness of the educational program about brucellosis among blood donors. Methodology: Quasi experimental design was used. This study was carried out at the main University hospital's blood bank in Assiut. The studied sample selected by simple random sample who was 300 blood donors. An interview questionnaire was used which included their socioeconomic characteristics. Also, pretest was done by questions regarding knowledge, attitude and their reported practices about brucellosis to assess the effectiveness of the educational program through immediate posttest. Blood sample was collected from all blood donors and analyzed by the enzyme linked immunosorbent assay (ELISA) test to detect the positive cases Results: According to their residence all the studied sample from rural areas. The prevalence of brucellosis was 16 % among them. Regarding knowledge of studied blood donors about brucellosis, in pre test the mean score was 10.37±5.20 while after implementing the educational program the mean score increased to 27.37±2.37 with highly significant differences (P value= <0.001).The mean score of reported practices and attitude were(1.9±1.14, 13.31±2.05) respectively. Conclusion: Brucellosis is a community health problem in Assiut Governorate. The blood donors' knowledge, attitude and practices were mostly poor and after completion of the program a significant improvement were achieved in the blood donors' knowledge. Recommendation: Further researches are needed to update the individuals' knowledge, positive attitude and appropriate practices about brucellosis.