Laparoscopy is a surgical procedure that allows for visualization of the abdominal cavity through a small incision. The aim of this study was to assess the effect of nursing Intervention Protocol about laparoscopic urological surgery on nurse‟s Performance and Patient outcome. Research design: A Quasi experimental research design was used to conduct this study. Setting: The study was carried out at urological operating room in urology and nephrology hospital. Sample: All available nurses (17) and 60 patients were included in this study. Tools of data collection were nurse‟s knowledge assessment questionnaire method, nurse's practice observational checklist, and Patient assessment sheet. Results: A positive correlation between nurses‟ knowledge and practice regarding urologic abdominal laparoscopic surgery. There was a statistically significant difference between both times of follow-up regarding incidence of complications. Conclusion: The nurse‟s knowledge and performance about laparoscopic surgery were limited. Recommendations: Improve level of professional nurses „performance through attending scientific meetings, conferences and well-structured training programs should be offered to the nurses for maintain high-quality of care.