Background: Nurses practitioners are so multidimensional, flexible, creative, and organized that developing and operating a business is a natural fit for many. Combine all this with a creative idea to solve a problem or fit a particular niche, and can got some valuable components to starting a small business. Aim: This study aimed to assess the effect of entrepreneurship orientation program on entrepreneurial intention among future nurses. Subjects and Methods: Quasi-experimental research design was adopted to conduct this study. The study was conducted at Suez Canal University Hospitals. The study participants were (76) nursing interns and (37) newly graduate baccalaureates nurses during the academic year 2018-2019. Tools: Entrepreneurial Intention Questionnaire was used for data collection. Results: Based on the participant`s responses, the results revealed that there was a significance (P=0.000*) improvement from the pretest to the post to the follow-up. Conclusion: The highest percentage of participants had the intention to start their own private practice. This meant that the entrepreneurship orientation program affects the entrepreneurship intention positively. Recommendations: The findings provided valuable insight for higher education institutes to design their curricular in such a way that further the self- efficacy of entrepreneurial actions and positive attitude on entrepreneurship.