Background:- Venipuncture is the commonest painful invasive procedures that usually applied for children, this process keep the children stressful and frightening. So this study aimed to examine the effects of balloon inflation and cough trick method on pain intensity during venipuncture among children. A quasi experimental design was used in this study. Subjects included 90 school age children were recruited from emergency unit as well as medical and hematology units in Assuit University Children's Hospital. They were selected randomly and divided into three groups: balloon inflation group, cough trick group and control group. Two tools were used for gathering necessary data which were a structured questionnaire and Facial Pain Scale- Revised (FPS- R scale). Results of the present study revealed that the majority of studied children have mild pain in study group I & II and more than two thirds have moderate pain in control group, both methods of intervention were effective in pain relief (p-value ≤ 0.000), but balloon inflation was slightly better. Conclusion balloon inflation and cough trick were effective in reducing pain intensity during venipuncture. Therefore, it is recommended to use an educational training program for nurses about using balloon inflation and cough trick as non- pharmacological intervention to reduce pain among children