Background: Human fertility affected by many factors as nutrition (unbalanced nutrition and unhealthy diet) be together to affect reproduction security in women. Evidence suggests that nutrition can play an important role in altering fertility-related outcomes in both men and women. Aim: this study aims to assess level of knowledge about nutrition that increases fertility in infertile women, and evaluate effect of educational intervention about nutrition on infertile women's knowledge in preconception period. Methods: Quasi experimental (pre – posttest) research design was carried out in this study. It was conducted at infertility outpatient clinic women's health Hospitals, Assiut University, Egypt. Sample included 100 infertile women. Structured interview questionnaire was used and included two parts: Sociodemographic data and questions to assess knowledge regarding fertility's nutrition. Results: There are statistical significant differences between pretest and posttest regarding total knowledge of infertile women about nutrition, P-Value is 0.001. Conclusion: Educational program about nutrition that may increase fertility is very important to enhance infertile women's knowledge regarding nutrition that may increase and decrease fertility. Recommendations: implementing a continuing educational program for women including counseling skills about importance of fertility nutrition during preconception period.