There is no doubt that hyperbilirubinemia is one of the most common diagnoses in the neonatal period; it is estimated to occur in 60% of term newborns in the first week of life. Aim: This study aimed to assess nursing practice regarding neonates with hyperbilirubinemia. Design: Descriptive research design was used to conduct this study. Subjects and method: A convenience sampling of 40 nurses caring for neonates with jaundice who admitted to neonatal intensive care unit of Assuit University Children Hospital. Setting: This study was conducted at Neonatal Intensive Care Unit of Assuit University Children Hospital. Tools: Two tools were used to collect the necessary data, a maternal & neonatal personal data sheet and observation checklist sheet. Results: It was found that all of the studied nurses (100%) had satisfactory level of practice related to weight, and one quarter (25%) of them had satisfactory level of practice related to skin care, while only (2.5%) had unsatisfactory level of eye care, diaper care and gavage feeding and there was no statistical significant relation between nurses level of practice, and their marital status, residence, age, years of experience and educational level. Conclusion: There were no statistically significant relationship between nurses' level of practice and their personal characteristics. Recommendations: Provideing nurses with procedure manual hand books containing all necessary information about nursing procedures related to quality of nursing care for neonates with hyperbilirubinemia will be benficial.