Abstract Background: Critically ill patients are at risk of resistant infection for several reasons, including, abnormal immune systems, and invasive procedures. Assessment of infection control designed to protect healthcare team and patients from exposure to infected blood and body fluids by applying the principles of infection prevention. Aim: The study identify to assess infection control precautions among health care team in intensive care units. Design: descriptive research design utilized in this study. Setting: The study carried out in intensive care units at Main Assiut university hospital. Subjects: The study comprised assessment of 295 staff from intensive care team. A total of (31) physicians, (202) nurses and (62) workers participated. Tools: two tools utilized to collect data pertinent to the study: Tool I: - Assessment health team questionnaire for infection, Tool II: Their information infection control precautions: (observational checklist). Methods: the study conducted through preparatory, and implementation phase for assessing after practice about infection control precautions. Results: The current study revealed that there was significant statistical difference between health team assessed adequacy of infection control precautions (61.3%, 94.6%) respectively their practice with P-value (p < 0.000*). Conclusion: Based on findings of the current study, it can be concluded that satisfactory practice level regarding infection control precautions many majority of nurses versus physicians. Recommendation: Continuous updating knowledge and practice of health care team through inservice educational programs regarding infection control precautions.