Knowledge of infertile couples about Assissted Reproductive Technology is an essential parameter to improve the infertility treatment and conduct it cooperatively Aim of the present study: was to assess knowledge and attitude of infertile couples about Assisted Reproductive Technology Subject and Methods: A descriptive study design was carried out in the outpatient infertility clinic at Women's Health Hospital Assiut University, Sample: On hundred infertile couples were recruited for this study Tools: structured interview schedule about demographic data, infertility history, and several relevant variables was used to assess the knowledge and 5 point Likert scale to assess the attitude of the infertile couples regarding Assisted Reproductive Technology Results: Eighty one percent of infertile couples had inadequate knowledge and positive attitude regarding Assisted Reproductive Technology represents eighty five percent with no statistical significant differences between the knowledge of infertile couples and their socio-demographic characteristics and couple's Attitude related Assisted Reproductive Technology. Conclusion and Recommendations: infertile couples have inadequate level of knowledge towards Assisted Reproductive Technology. So the nurses in infertility clinics should premise on health education and counseling about Assisted Reproductive Technology.