Background: The aim of this study was to investigate the relationship between professional identity and occupational culture among faculty staff (87) at the faculty of Nursing -Ain shams university. A descriptive correlational design was used in this study, Data collection tools 1- Measure the Teacher Identity sheet and 2- Occupational culture scale, Results showed a statistically significant difference relating to culture domains of staff regarding their qualification, position and departments. And there is a statistically significant difference between services and their relationship and responsibility; Conclusion: The study findings demonstrated linkage between professional identity and occupational culture among study subjects. and There is a statistically significant difference in relation culture domains of staff regarding their qualification, position and departments, on the 0ther hand, there is a statistical significance difference between services of occupational culture domains and desired outcomes of professional identity of studied staff This study Recommended that the professional identity is an area that has not been researched in any great depth among the professions let alone in higher education.