Background: Injuries among school students are considered as one of the most serious health problems. Aim of this research: To assess knowledge and practice of the school trainers and students' knowledge regarding sports injury and its safety measures Research design: A descriptive design was used in this study. Setting: The study was conducted at four schools; which included all preparatory and secondary sports and military schools at Assiut Governorate. A convenient sample was used. The total number of school trainers and students was (656). Two tools were used (1) Self administrative questionnaire for trainers and students, (2) Trainers observation check list. Results: Finding revealed that more than half of the studied trainers aged more than 40 years and the majority of them were males. 51.1% of the trainers had < 20 years' experience and 33.3% of them were attended training courses about sports injury. Finding cleared that 36.3% of the studied students aged 14 to < 16 years and 79.2% of them were males. There was statistical significant difference between students' personal characteristics and their total score of knowledge. Conclusion: More than half of school trainers had good knowledge regarding to sports injury safety measures and most of trainers had adequate level of practices; while about half of students had poor knowledge regarding to sports injury safety measures. Recommendation: The study recommend establish continuous educational program for trainers and students to improve their knowledge and practices.