Background: Food Allergy is an abnormal response to a food protein triggered by the body's immune system. Aim of the study: to assess knowledge and attitudes of students about food allergy at Assiut University. Research design: descriptive research design was used in this study. Setting: The study was conducted in 4 randomly selected faculties at Assiut University (Faculty of Social Work, Computers and Information, Arts and Veterinary Medicine). Total sample was 931 students. Two tools was used (first tool) A structured self-administered questionnaire. (second tool) likert scale to assess attitude toward food allergy. Results: Finding revealed that more than two fifth of them were 20-21 years old. About three quarter of studied students had poor score of knowledge, while only 4.9% of them had good score of knowledge. The majority of studied students had positive attitudes toward food allergy. Conclusion: The study concluded that there were lack of knowledge about food allergy. There was a statistical significant difference between sex, mother's education and their knowledge. Recommendations: The study recommended that develop plan, and implement health education programs for university students about food allergy.