Aim: To test the efficacy of nursing role in implementing new protocol for management iron deficiency anemia in pregnancy. Design: A prospective interventional longitudinal study. Setting: Conducted at Women's Health Assiut University Hospital. Sample size: 60 cases divided into two groups: (A) received iron supplementation with health education and (B) received iron supplementation without health education. Results: Reflects positive outcomes of oral lactoferrin fizzing with a statistically significant difference when compared with other two different routes (Total infusion dose and oral tablets) of iron supplementations. Conclusion: Oral iron supplementation plus proper health education during implementing of the new protocol for management iron deficiency anemia in pregnancy, confirmed that oral iron supplementations in this study has a similar effect as parenteral rout in improvement the hemoglobin, MCV and MCH which reflect the importance of health education introduced by nurse. Recommendation: Increase awareness of pregnant women about importance of iron supplementation accompanied with good sources of nutrition and encourage health facilities to detect IDA with pregnant women earlier during ANC visits.