Background: there is a question with obstetricians and health staff whether implemented evidence-based guideline, can affect labor outcomes. This study was conducted to implement evidence-based guideline practice during the third stage and all stages of labor will affect in reducing postpartum hemorrhage and giving better labor outcomes. Aim of the study: to determine effect of an evidence-based guideline on caring a woman during the third stage of labor and evaluate the labor outcome of evidence-based intervention (EBI). Materials and methods: Design: A quasi - experimental design. Tools structured interviewing sheet including socio-demographic data. Observational sheet including nursing practice, and Follow up sheet including assess blood loss. Setting: data collected from delivery unit, Minia University maternity Hospital. Sampling: simple random sampling, one hundred included in the study; divided into two equal (control and study group). Results: the study revealed that there were mean of blood loss after 1, 2, 3 hours of delivery can lower in the study than control group. On the other hand, There was a highly significant relationship between maternal outcome and their neonatal apgar score level (P= 0.001). Conclusion and Recommendation: further researches are still needed to implemented guideline of nursing practices for childbirth and measures could be decreasing intra-postpartum hemorrhage and others complication.