Health care staff working in the blood banks and transfusion services are at risk of exposure to pathogenic organisms in blood.The study aimed to assess knowledge and practice of health team toward infection control in the blood banks at Assuit city.A descriptive research design was used in the study.The total number of this studied sample was 129.Two tools was used in the study; tool 1:Self-administrator questionnaire tool 2: observation check list. Results the present study found that slightly more than two-fifths of studied sample(41.1%) had unsatisfactory knowledge about infection control. While slightly more than three-quarters of them(75.2%)had unsatisfactory performance concerning infection control measures in the blood banks. There was a positive correlation between knowledge and performance of studied sample(r=0.132).While there was a negative correlation between age and years of experience with knowledge about infection control among the studied sample(r=- 0.031, -0.133 respectively).It concluded that the studied sample had poor knowledge and performance toward infection control in blood bank.Recommendations providing training program about the infection control measures and it's importance for the new employees in the blood banks. Providing continuing training courses about infection control for all health team.Distribution of a simple guide book about the infection control.