Nurses' professional values play an important role in the degree of burnout that the nurses may expose in their workplace. This study aimed to find out the relationship between professional values and burnout among nurses. Methods: research design: Descriptive correlational research design. Setting: this study carried out at the main Assiut University Hospital. Subjects:  The sample consists of 479 nurses working in Assuit University hospital in general and specialized medical, surgical and intensive care units. Tools of study: The study tools consisted of three tools which included: Demographic data sheet, Nurses Professional Values Scale, andMaslach's Burnout Inventory scale. Results: A High percent of the studied nurses reported that professional values were the most important in nursing practice. A High percent of the studied nurses who graduated from the secondary technical school of nursing had high level of burnout. Conclusion: There was a positive correlation between nurses' professional values and burnout. Recommendation: Generalization of Egyptian Code of Ethics for Nursing Profession to all governmental and private hospitals at Egypt and Providing adequate staffing and flexible scheduling for nurses to suite the personal and social stabilization through regulating the rotating shift.