Background: Breast cancer is the most common cancer among women in developed and developing countries. Reproductive factors are associated with higher risk for breast cancer. Aim of the study: To clarify the effect of reproductive, menstrual and familial factors on the risk of breast cancer, pre and post menopause. Subjects and Methods: A case-control study design was used in this study and convenient sample for the women selection, the sample comprised 300 women that divided into two main groups, 150 for each group, Study group and control groups. Completing questionnaire from all women. Results: The study revealed statistically significant associations between breast cancer and age, family history, late age at menopause (p<0.001), early age at menarche, (p<0.009), longer cycle duration (p<0.001), contraceptive pills (p<0.017), also the result revealed that there was no statistically significant between these factors and breast cancer regarding menopausal status. Conclusion: the findings of the present study support that there is a statistically significant relation between some of reproductive, menstrual, familial factors, and breast cancer. Recommendation: Health education programs regarding breast cancer, risk factors and early detection. Nurses should provide practical training in breast self-examination, especially for those women who are at higher risk of breast cancer.