Health promoting behaviors and psychosocial well being are important determinants of health status and quality of life. Aim of the study: Assess the health promoting lifestyle of nursing student's at Assuit University. Methods Across-sectional descriptive research design carried out in Assiut University included 1045 students from faculty of nursing (430 students), technical institute of nursing (323 students) and secondary nursing school (292 students). Tools: Data collected by using two tools; tool (1): interview form consisted of two parts; first part included sociodemographic data. The second part included assessment of anthropometric measurement was used to record weight and height measurements for all students then calculate BMI. Tool (2): Health Promoting Life Style Profile (HPLP) scale, the scale consists of 48 items. Result: 39.1% of the studied students were their age more than 17- years. The mean score of health promoting life style among studied students is higher in self-actualization and health (38.12 ± 7.46 and 25.07 ± 6.51) respectively. Conclusion: The mean score of health promoting life style among studied students is higher in self-actualization and health (38.12 ± 7.46 and 25.07 ± 6.51) respectively. Recommendation: Increasing Health education programs for students about how to maintain health promote and achieve maximum level of well-being and avoid risky behaviors.