Background: Hepatitis C virus (HCV) is an illness where changes happen slowly, so it is possible for patients to live healthily. The aim of the study: was to suggest nursing guidelines for patients with HCV based on their identified needs through: Assessing patients level of knowledge regarding HCV disease, tips that promote healthy living with HCV disease as well in relation to the preventive measures to avoid the spread of HCV infection, assessing patients informational health needs, medical and supportive needs. Study design: descriptive research design utilized to conduct this study. Subjects and Methods: a purposive sample of 200 adult patients newly diagnosed with HCV within 6 months, the study was conducted at Hepatitis viruses' outpatient clinic at Assiut University Hospital. Tools: "Interview questionnaire sheet". Results: the majority of patients had unsatisfactory knowledge about HCV, healthy living with HCV and prevention of the spread of infection (97.5%, 91.5%, 63.5% respectively). Patients newly diagnosed with HCV need information regarding HCV and healthy living. Conclusion: Patients newly diagnosed with HCV had unsatisfactory level of knowledge about HCV and healthy living with it. Recommendation: Provision of guidelines booklet is of great importance for the patients.