Although the public is increasingly concerned about food-related risks, the rise in food poisoning cases suggests that people still make decisions about food safety that are less ideal from a health and safety perspective. The aim: To assess the food safety knowledge and practices among working and non-working women in Damanhour. Subjects & Methods: Research design: Cross-sectional descriptive study. Sample: of 200 rural women were recruited (working and not working) from 2 villages in Damanhour. Tool: Structured interview questionnaire was used to collect data from food prepared women to identify their food safety knowledge and practices. The study conducted from August 2014 to December 2014. Results: Nearly 60% of the sample had a university education, nearly 40% of them were above 30 and less than 40 years old. Moreover, the majority of them had unsatisfactory food safety knowledge. The mass media was one of the main sources of their information. Conclusion: Significant differences was observed between women (working and nonworking) and their food safety knowledge and practices. Recommendation Develop and implement effective food safety educational programs that result in safer food handling practices of women in relation to all aspect of food safety.