Reproductive health is an essential part of human health and development for women, particularly during their reproductive years. The aim of the study: To evaluate the effects of a designed educational program about reproductive health and on nurses‘ knowledge. Design: A pre- post- test, non- equivalent design was used. Setting: The study was conducted at 16 primary health care units and Maternal and child health units in Qena governorate. Sample purposive Convenient samples of 100 nurses working in the selected setting. Tools of data collection1-Interview sheet to collect demographic data about nurses age ., Residence, and years of experience 2--: A structured test that was developed by the investigators to collect nurses knowledge about reproductive health pre and post test. The program: The nurses were divided into 4 groups (25 nurses in each). The training program was conducted over a period of 12 days; 3 days per group for 3 sessions per day for three months and post-test after six months covering all information and practices of the program. Results: the improves the level of knowledge of adequate level increased from 28% before the health education program to 98% and a significant difference in knowledge. Between Nurses working for 10 years or more than those working less than 10 years Conclusion: The education program markedly improves the level of knowledge of those with adequate level increased from 28% before the health education program to 98% after the program.