Upper respiratory tract infections (URTIs) are common in children and represent a significant cause of antibiotic misuse which contributes to the development of antibiotic resistance. Aim of the study: was to assess knowledge, attitudes and practices of parents related to antibiotic use and misuse among children with upper respiratory tract infections Descriptive research design was used to conduct the study at the pediatric Out-patient Chest Clinic in Assiut University Children's Hospital. Convenience sample was selected that included 420 parents with their children suffering from URTIs from December 2014 till February 2015. A questionnaire sheet was used to collect the data. Results of the present study revealed that 72.9% of the parents were females; the mean age was 35.4+9.3, 34.5% of the parents were having poor knowledge about the antibiotic use and misuse. Thirty percent think that antibiotics are safe, 48.1% of them reported that are a little informed about judicious antibiotic used. 41.5% pay a little attention to the possible side effects of antibiotics. Conclusion: More than one third of parents were having poor knowledge about the antibiotic use and misuse; and nearly half of them were having little information about judicious use of antibiotic which reflected on their self reported attitude and practice. Recommendation: An educational intervention for parents include educational materials about judicious use of antibiotic is needed.