Infections account for the second leading cause of mortality among patients with end-stage renal disease. Many of these infections are due to sepsis, primarily arising from the vascular access site. The aims of this study: were to assess nurses' knowledge and practice about infection control during hemodialysis, design of an educational nursing program, implementing & evaluating the effect of this educational nursing program on infection control in hemodialysis. Setting: This study was conducted in hemodialysis unit at Om El-Kosoar, Assiut University Hospital. Subjects: purposful sample of 55 nurses of both sexes, age ranges from 18 to 60 years. Tools: Self-administed questionnaire sheet, observational checklist and educational nursing program. Results: there was a highly significant difference and improvement as regard knowledge level and practice score for nurses about infection control pre dialysis, intra dialysis and post dialysis after implementing the nursing education program. Conclusion: Poor nurses' knowledge and practices pre program, improve nurses knowledge and practices about infection control post program. Recommendation: Continue nursing training sessions about infection control in hemodialysis unit to reduce the incidence of infection.