Aim of the Study: To assess awareness, knowledge and practice about injection safety among nurses working in medical and surgical units at Assiut ministry of health hospitals. Patients and method: Descriptive cross-sectional research design was utilized in this study. Sample: 500 male and female nurses. The study was conducted in medical and surgical units at five hospitals in Assiut ministry of health hospitals. Tools: Two tools used in the study :Tool (I) Self-administered questionnaire sheet consisted of three parts: (1) demographic characteristics of the nurses, (2) immunization and needle stick incidence among nurses, (3) assessment of nursing awareness and knowledge about injection safety. Tool (II): Observational check-list sheet. Results: Nearly all nurses 98.6% had a good awareness level and more than half had a satisfactory knowledge score and good practice score (69.4% and 63.4% respectively) regarding injection safety. Conclusion: Majority of the nurses had a good level of awareness regarding injection safety but less than half of them had unsatisfactory knowledge and the practical injection safety precautions were not followed properly. Recommendation: Nurses in need for in-service training programs and refreshing courses to improve their awareness and knowledge about injection safety which will reflect on their practice while working with patients.