Aim of the study: the study was aimed to evaluate the effect of teaching program on nurse`s performance regarding Guillian Barre Syndrome patients at neurological Care Unit at Assuit University Hospital. Research design: Quasi experimental research design was utilized in the study. Subject and method: the study was carried out on a convenience 30 nurses who participating in Guillian Barre patient care. One tool have been utilized to gather the information, tool I structure questionnaire divided into three parts: part 1 demographic characteristics of the nurses, part 2 pre/post knowledge assessment questionnaire, part 3 pre/post practice observational checklist. Tool had been utilized in 3 phases; preparatory, implementation and evaluation phase,with applying of teaching program for nurses about knowledge and practice about the care of Guillain-Barre syndrome patients. Results: The frequent of nurses had unsatisfactory whole expertise pre teaching program (12.56±2.45) compared with post teaching program (21.5±1.52) the suggest score had been improved significantly (P=0.000), additionally the majority of nurse`s practice have been inadequate pre teaching program (127.93±44.58) compared with post teaching program (248.5±9.11). Conclusion: the find out about used to be illustrated that the teaching program leads to improvement in nurse`s knowledge about Guillain-Barre syndrome patients. Recommendation: Continuous nursing training and preparing programs on neurological intensive care unit should be well planned at Assiut university hospital.