Currently hospitals are spending much money in Information Systems (IS) to enhance quality of care, efficiency and safety. Aim: The study aimed to assess the disposition of nursing staff toward using information systems. Research design: A descriptive design was carried out in this study. Setting: The study was performed at Intensive care units of Assuit University Hospitals (Chief Assuit University Hospital, Women Health Hospital and Pediatric University Hospital) Subjects: A convenient sample (250) of nursing staff from the previous setting. Tools of the study: Two tools were used, the first one was: A self-administered questionnaire about Knowledge of nursing staff about using information systems and the second one was: A self-administered questionnaire about nursing staff attitudes toward using information systems Results: The nursing staff have good knowledge and positive attitudes toward using information systems and there was a positive relationship between all variables of the study participants' knowledge and total score of attitudes toward using information systems with great statistically significant difference. Conclusion: The nursing staff have good knowledge and positive attitudes toward using information systems. Recommendation: The study recommended that, Hospital administration should work to implement a health information system for the hospital.